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Quantitative Determination of β-Asarone in Calamus by High-Performance Thin-Layer Chromatography


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Quantitative Determination of β-Asarone in Calamus by High-Performance Thin-Layer Chromatography

Valeria Widmer, Anne Schibli, and Eike Reich

CAMAG Laboratory, Sonnenmattstrasse 11, 4132 Muttenz, Switzerland
Abstract

A quantitative HPTLC method for determination of β-asarone in Calamus rhizome was developed and validated. The method is suitable for proper identification of Acorus calamus. Using caffeine modified silica gel as stationary phase and toluene – ethyl acetate (93:7 v/v) as mobile phase β-asarone is baseline separated from its isomer α-asarone. Scanning densitometry with absorption measurement at 313 nm allows specific, accurate and precise quantitation of β-asarone. The working range of 25 to 300 ng absolute of the target substance is sufficient to establish whether a given sample passes the limit test of max 0.5% as required by the Swiss Pharmacopoeia.


Key Words

α-asarone, β-asarone, Calamus, quantitation, densitometry, validation, HPTLC



Journal of AOAC International, Volume 88, No. 5 (2005), p. 1562-1567


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