Ana səhifə

Fukai Frank Bao cv general Information Professional Title

Yüklə 70.91 Kb.
ölçüsü70.91 Kb.

Fukai Frank Bao CV
1. General Information

Professional Title:

Fukai Frank Bao, MD/PhD

1. Professor, and chairman, Department of

Microbiology,parasitology and Immunology

2. Director, The Institute for Tropical Medicine

3. CTO, Quality Control Station of Experimental Animals

Kunming Medical University, Kunming, China

4. Director, Pan-Asian Centers for Tropical Infectious Diseases

Contact Information:

Address:Department of Microbiology and Immunology,

Kunming Medical University

191 Renmingxi Road, Kunming 650031, China

Tel: 86-871-5314539

Fax: 86-871-5314539



1980~1985 MD, Kunming Medical College, Kunming, Yunnan, China

1985~1987 MS, Kunming Medical College, Kunming, Yunnan, China

1987~1990 PhD, Kunming Medical College, Kunming, Yunnan,


2002~2005 Postdoc, School of Medicine, Yale University, New Haven, CT, USA

2009~2009 Visiting professor, Mahidol University and The Prince of Songkla University, Thailand

2011-2011 Visiting professor, Mahidol University and The Prince of Songkla University, Thailand

Professional Membership:

Vice-President, International HUA-XIA Medical Society

Member,WHO Stop TB Partnership

Member, American Society for Microbiology (ASM)

Member, New York Academy of Sciences (NYAS)

Member, American Spirochete Research Community

Member, Chinese Society of Microbiology, Beijing, China

Committee member, Chinese Committee of Clinical Microbiology

Chairperson, Subcommittee of Intracellular Pathogens

Member, Chinese Society of Immunology, Beijing, China

Journal Editors And Reviewers:

Journal of Rapid Methods and Automation in MicrobiologyReviewer, Wiley

Vector-Borne and Zoonotic DiseasesReviewer, USA

Journal of Entomology and Nematology ( Co-editor)

FEMS Immunology & Medical Microbiology (Reviewer)

Journal of Bacteriology ResearchAssociate editor

Journal of Yeast and Fungus Research (co-editor)

The Journal of Infection in Developing Countries (JIDC,Italy)

Journal of Developmental Biology and Tissue Engineering ( Reviewer )

Clinical Reviews and OpinionsMember of editorial board

Journal of Pharmacognosy and Phytotherapy(Reviewer)

Journal of Clinical Immunology and ImmunopathologyMember of editorial board

China Tropical Medicine ( Editorial Board member and reviewer,China)

Chinese Journal of Medical and Pharmaceutical Sciences

( Editorial Board member and reviewer,China)

Chinese Journal of Clinical Laboratory Science(Reviewer, China)

Journal of Clinical Medicine and Researchreviewer

Journal of Infectious Diseases and immunityMember of editorial board

Journal of AIDS and HIV Researchreviewer

Journal of Cancer Res and Exp Oncologyreviewer

Journal Of Dentistry And Oral HygieneReviewer

Journal Of General And Molecular Virologyreviewer

Journal of Developmental Biology and Tissue Engineering (Reviewer)

Progress in Physiological SciencesBeijing, China

Genetics and Developmental BiologyBeijing, China

Life SciencesShanghai, China

Chinese Journal of Epidemiology(Member of editorial board, Beijing, China

Research Opinions in Animal & Veterinary Sciences (ROAVS)editorial board member

Journal of Pharmacognosy and Phytotheraphy

2. Lab’s Research Highlight:

(1) My laboratory is much interested in arthropod-borne pathogens and its infections to the mammalian hosts.

Tropical arthropod-borne infectious diseases remain an major international public health threat. My laboratory focuses on several kinds of arthropod-transmitted diseases:

a. Borrelia burgdorferi, a species of the spirochete, which causes Lyme disease.

b. Anaplasma, mainly Anaplasma phagocytophilum, an obligate intracellular bacterium that persists within neutrophils, which causes human granulocytic anaplasmosis and is primarily transmitted by Ixodes ticks.

c. Finally, richettsiaes, which are expanding their territory throughout the world.

(2) We put hard efforts on Mycobacterium tuberculosis, tuberculosis epidemiology, anti-tuberculosis natural products, TB immunology, epidemiology, molecular typing, and pathogen-host interaction.

Tuberculosis(TB), An ancient disease, remains one of the major causes of disability and death worldwide. In 2006, 9.2 million new cases of TB emerged and TB killed 1.7 million people. My lab is examining TB at its fundamental levels through researches aimed at developing faster diagnostic tools, better prevention and treatment strategies.

Tuberculosis, or TB, is an infectious bacterial disease caused by Mycobacterium tuberculosis, which most commonly affects the lungs. It is transmitted from person to person via droplets from the throat and lungs of people with the active respiratory disease. In healthy people, infection with Mycobacterium tuberculosis often causes no symptoms, since the person's immune system acts to “wall off” the bacteria. The symptoms of active TB of the lung are coughing, sometimes with sputum or blood, chest pains, weakness, weight loss, fever and night sweats. Tuberculosis is treatable with a six-month course of antibiotics (DOTS). A single sneeze can release up to 40,000 droplets. Each one of these droplets may transmit the disease, since the infectious dose of tuberculosis is very low and the inhalation of just a single bacterium can cause a new infection.

About 90% of those infected with Mycobacterium tuberculosis have asymptomatic, latent TB infection (sometimes called LTBI), with only a 10% lifetime chance that a latent infection will progress to TB disease. However, if untreated, the death rate for these active TB cases is more than 50%. TB infection begins when the mycobacteria reach the pulmonary alveoli, where they invade and replicate within the endosomes of alveolar macrophages. The primary site of infection in the lungs is called the Ghon focus, and is generally located in either the upper part of the lower lobe, or the lower part of the upper lobe. Bacteria are picked up by dendritic cells, which do not allow replication, although these cells can transport the bacilli to local (mediastinal) lymph nodes. All parts of the body can be affected by the disease, though it rarely affects the heart, skeletal muscles, pancreas and thyroid.

Tuberculosis is classified as one of the granulomatous inflammatory conditions. Macrophages, T lymphocytes, B lymphocytes and fibroblasts are among the cells that aggregate to form a granuloma, with lymphocytes surrounding the infected macrophages. The granuloma functions not only prevent dissemination of the mycobacteria, but also provides a local environment for communication of cells of the immune system. Within the granuloma, T lymphocytes secrete cytokines such as interferon gamma, which activates macrophages to destroy the bacteria with which they are infected. Cytotoxic T cells can also directly kill infected cells, by secreting perforin and granulysin.

Importantly, bacteria are not always eliminated within the granuloma, but can become dormant, resulting in a latent infection. Another feature of the granulomas of human tuberculosis is the development of cell death, also called necrosis, in the center of tubercles. this necrosis has the texture of soft white cheese and was termed caseous necrosis.

(3) Our research is also focused on Molecular epidemiology and ecology of tropical vector-borne pathogen infections, and characterizing tropical vector-borne pathogens, particularly tick-transmitted rickettsial disease agents, including Ehrlichia chaffeensisAnaplasma phagocytophilum and mite-transmitted Orientia, and also mosquito-transmitted virus infection, such as dengue virus, Chikungunya virus.

We use the tools of field epidemiology, molecular biology, immunology, animal models and cell culture systems. E. chaffeensis is the agent causing human monocytic ehrlichiosis (HME). It also infects dogs, white-tailed deer, goats and coyotes. These closely related pathogens remain in both arthropod and vertebrate hosts for long periods of time. This strategy may aid them in their parasitic lifestyle. Determining the molecular basis for persistence by these bacteria of the genus Ehrlichia will be important in order to accomplish our long range goals of developing effective methods of control against these tick-borne pathogens. Our research is focused on understanding the pathogen evasion mechanisms. Specifically, we study to define the protective host-immune response needed to eliminate the infection. We also study to define how these rickettsiales evade host responses in both vertebrate and tick hosts in order to support their life cycle.

3. Lab Facilities and Equipments:

Our well-equipped lab system includes 1 high-quality immunology/cell culture room (80m2), 1 biosafety level-2 microbiology room (80m2), and 2 biosafety level-3 labs (200m2). The lab system has been well equipped with biosafety cabinets, ultra-clean hoods, ultra-speed frozen centrifuges, ultra-low freezers, Gel Image - Forming System, PCR machines, DNA-RNA-protein electrophoresis systems, Western blot machine, fluorescence microscope, ELISA reader, CO2 cell incubator, visible-UV spectrophotometers, common incubators, and other basic research tools.

4. Experimental Technology and Skills Platfrom:

Our lab system has strong abilities for technical services,including:

1. In vivo and in vitro activity screening of bioactive products and natural products against bacteria,fungi,infectious diseases, and tuberculosis

2. In vivo and in vitro activity screening for immuno-regulation activities of bioactive products and natural products

3. Systematic classification and identification of new bacteria and fungi; Isolation and identification of bacteria and fungi in various samples

4. Emergency response and consultation of sudden tropical infectious diseases

5. PCR, RT-PCR, Western blotting, ELISA techniques

6. Extraction and purification of DNA, RNA, and protein 7. Cell culture skills, and animal experiment skills

5. Lab Members:

Our lab hosts a strong active multi-disciplinary group of 30 members, their research fields are involved in microbiology, immunology, molecular biology, field epidemiology, infectious diseases, vector biology, and bioinformatics, etc. We also recruit graduate students and short-time training foreign students every year.

6. Collaboration and Information Communication:

1. WHO Stop TB PartnershipGeneva

2. TB Communities, Global Health Delivery Project

3. Southeastern National Tuberculosis Center

University of Florida, USA

The University of Southern Mississippi,USA

Yale University School of Medicine,USA

5. McGill University, Canada

6. Prince of Songkla Unversity, Thailand

MahidolUniversity, Thailand

7. National University of Moscow, Russia

8. The Institute of Tropical Medicine, Nagasaki University,

Nagasaki, Japan

9.Ditan Hospital, Beijing, China

People’s Hospital, Beijing, China

The Institute for Pathogen Biology, Chinese Academy of Mdecine, Beijing, China

CDC,Chengdu Military Region, Chengdu, China

The Yunnan Institute of Endemic Disease prevention and Control, Dali, Yunnan, China

The Third Hospital of Kunming (Kunming Hospital for Infectious Diseases, Kunming, Yunnan, China

7. Recent Main Publications(2004~)

1) Pal U, Wang P, Bao F(First authors with equal contribution), Yang X, Samanta S, Robert Schoen , Wormser GP, Schwartz I, and Erol Fikrig. Borrelia burgdorferi basic membrane proteins A and B participate in the genesis of Lyme arthritis. J Exp Med. 2008; 205: 133-141.(SCI Impact factor:15.6, 2008)

2) Utpal Pal, Xin Li, Tian Wang, Ruth R. Montgomery, Nandhini Ramamoorthi, Aravinda M. deSilva, Fukai Bao, Xiaofeng Yang, Marc Pypaert, Deepti Pradhan, Fred S. Kantor, Sam Telford, John F. Anderson, and Erol Fikrig. TROSPA, an Ixodes scapularis receptor for Borrelia burgdorferi. Cell.2004;119:457-468.(SCI Impact factor:32.4)

3) Nandhini Ramamoorthi, Sukanya Narasimhan , Utpal Pal, Fukai Bao, Xiaofeng F. Yang ,Juan Anguita, Michael V. Norgard, Fred S. Kantor, John F. Anderson, Raymond A. Koski and Erol Fikrig. The Lyme disease agent exploits a tick protein to infect the mammalian host.Nature.2005;436:573-577.(SCI Impact factor:32.7)

4)Yongli Zhang, Fukai Bao, Jianjian Hu, et al. Antimicrobial lignans and triterpenoids from Rostellularia prcumbens. Planta Medica. 2007; 73:1596-1599. (SCI journals)

5)Hengxing Liang, Fukai Bao, Xiaoping Dong , Rui Tan , Caijun Zhang , Qing Lu and Yongxian Cheng. Antibacterial Thymol Derivatives Isolated from Centipeda minima. Molecules. 2007; 12:1606-1613.(SCI jurnal)

6)Heng-Xing Liang, Fu-Kai Bao, Xiao-Ping Dong, Hua-Jie Zhu, Xiao-Jie Lua, Ming Shi, Qing Lu, and Yong-Xian Cheng. Two New Antibacterial Sesquiterpenoids from Centipeda minima. Chemistry & Biodiversity. 2007; 4:2810-2816. (SCI journal)

7)Ren-Qiang Mei , Qing Lu, Yan-Fen Hu, Hai-Yang Liu, Fu-Kai Bao, Yu Zhang, Yong-Xian Cheng. Three New Polyyne (=Polyacetylene) Glucosides from the Edible Roots of Codonopsis cordifolioidea. Helvetica Chimica Acta. 2008; 91:90 – 96.(SCIjournal)

8)Fengwei Bai, Tian Wang, Utpal Pal, Fukai Bao, L. Hannah Gould and Erol Fikrig. Prevention of lethal murine West Nile virus infection using RNA interference. Journal of Infectious Diseases. 191:1148-1154, 2005. (SCI journal)

9) Fukai Bao, Erol Fikrig. Experimental research on the coinfection with Borrelia burgdorferi and Anaplasma phagocytophilum. Journal of Tropical Medicine,2007;7:195-200.

10) Fukai Bao, Erol Fikrig. Coinfection with Borrelia burgdorferi and Anaplasma phagocytophilum inhibits the production of Th1 cytokines. China Tropical Medicine. 2007;7:1061-1063.

11) Fukai Bao. Research advances in the coinfection of tick-borne pathogens。China Tropical Medicine. 2007,7(1):112~114

12)Fukai Bao. Research progress in Borrelia burgdorferi and Lyme disease. Journal of Tropical Medicine. 2007;7:1125-1127

13)Fukai Bao. Systematic taxonomy of Rickettsiales. Chinese Journal of Zoonoses. 2007;23:1262-1264

14) Fukai Bao, Aihua Liu, Yu Zhang。In Vivo Anti-Tubeculous Effect of Tiankejun Capsule。Journal of Tropical Medicine,2006,6(3):975-977

15)Aihua Liu,Fukai Bao*,Jie Zhang, Jue Shu。Observation of the antibacterial effect of extracts from Herba Euphorbia humifusa on oral pathogenic bacteria in vitro. China Tropical Medicine. 2007 ,7 (12 ):2194,2217

16) Aihua Liu,Bixuan Li,Min Shi,Fukai Bao*. Terpenoids YK—1 and YK—2 shows anti-bacterial activities in vitro. Chinese Journal of Pathogen Biology. 2008, 3(3):176-177

17)Henxin Liang, Fuaki Bao, Xiaopin Dong, Qing Lu, Yongxian Chen*.Antibacterial Triterpenes from Centipeda minima.Yunnan Plant research. 2007 , 29 (4) : 479~482

18) Fukai Bao,Erol Fikrig. The pathogen-vector interactions during Borrelia burgdorferi transmission. China Journal of Vector Biology and control。2008, 19(3):264-268

19) Fukai Bao, Erol Fikrig. The Joint-specific Expression Profile of Borrelia burgdorfri in the Murine Hosts. Science and Technology Bulletin. 2008, 24(6):832-838

20) Fukai Bao*,Aihua Liu,Haibin Ma. Advances in pathogen biology and epidemiology of human anaplasmiosis. Journal of Tropical Medicine,2008,8(11):1193-1195

21) Fukai Bao*,Aihua Liu,Haibin Ma,Na Wu, Min Shi. Advances in the pathogenesis of Lyme arthritis. Chinese Journal of Pathogen Biology. 2009,4(5):380~382

22) Aihua Liu,Lin Chen,Caijun Zhang,Fang Chen,Min Shi,Liang Chen,Peiqing Shen,Ruwen Liu,Jianlin Jiao,Malin Li,Fukai Bao*.Touchdown PCR detected Borrelia burgdorferi in wild tree shrew. 2009,9(4):621~622

23) Fukai Bao*,Aihua Liu。Quantitative research of Borrelia burgdorferi burden in the murine model。Chinese Vector Biology and control。2009, 20(3):234~236

24)Aihua Liu,Fukai Bao*。Newly-found inhibitory cytokine—Interleukin(IL)-35。Chinese Journal Of Immunology. 2009; 25

25)Li Li,Fukai Bao*。Il-32 and its relations to inflammatiory diseases. Journal of Current Immunology. 2009,25:284~285

26)Fukai Bao*, Aihua Liu, Min Shi,Haibin Ma。Advances in Clinical symptoms, diagnosis, and treatment of human anaplasmiosis. Journal of Tropical Nedicine。2009;9: In press

27)Liang Chen,Fukai Bao*. Isoniazid-resistant genes and resistant mechanisms of Mycobacterium tuberculosis. Chinese Journal of Zoonoses. 2009;25(3):288~291

28)Fukai Bao*,Aihua liu,Chuanxian Chen。Epidemic trend, cause-consequence analyses,and control strategies. China Tropical Medicine. 2009 ,9(3):599~562

29)Aihua Liu,Fukai Bao*。Some important advances in innate lmmunity study. Chinese Journal of Nature. 2009;31(4); 218~222

30)Ai-hua Liu, Min Shi, Cai-jun Zhang, Xiao-jie Li, Xin Wang, Pei-qing Shen,Ma-lin Li , Fukai Bao*. . Domesticated tree shrew ( Tupaia belangeri ) could be infected with Serratia grimesii. Afr J Biotech. 2010; (SCI journal)

31) Qun XUAN, Fukai BAO*., Hongmei PAN,Aihua LIU 3*. Isolating Fungal Endophyte from Paris polyphylla Smith var. yunnanensis and Identifying Their Antibacterial Ability. Afr J Microbiol Res. 2010; (SCI journal)

32) BinXue Li, Fukai BAO*, Aihua LIU. Research progress on relationship between tumour necrosis factor-ɑ and tuberculosis. China Tropical Medicine. 2010;10(3): 370-372

33) ZHANG Caijun , SHI Ming , CHEN Fang , CHEN Liang , SHEN Peiqing, BAO Fukai*, L I Malin*. Isolation and identification of common bacteria in tree shrews. China Journal of Pathogen Biolog y. 2009;4(12): 899-900

34) BAO Fukai*, L IU Aihua. Progress in the study of Ehrlichia cha f f eensis and human monocytic ehrl ichiosis. China Journal of Pathogen Biology. 2009;4(12): 935-938

35) Dong Shasha,Bao Fukai,Lu Qing,Chneg Yongxian1*. GC-MS Analysis of the Chemical Constituents of Essential Oil from Hibiscus sabdariffa Linn. and Study on Antibacterial Activity. Journal of Dali University. 2009; 8(6):1-4

36) LIU Aihua, XUAN Qun, BAO Fukai. Progress of research on IL-32 and its relationship with tuberculosis. China Journal of Pathogen Biology.2010;5(3):215-217


( * correspondence author)

B. Scientific Books

1. Infection and Microbes (900 pages) Chendu: Chendu University of

Science and Technology Publishing House, 1995. (As the first vice


2. Medical Microbiology (400 pages, textbook for college student).

Shanghai: Shanghai Scientific and Technology Press, 1995. (As one of


3. Abbreviations and Terms in Immunology (324 pages). Peking: Academic

Press, 2002. (As one of authors)

Verilənlər bazası müəlliflik hüququ ilə müdafiə olunur © 2016
rəhbərliyinə müraciət