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Curriculum Vitae Dr. Kiran D. Pawar


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Curriculum Vitae


Dr. Kiran D. Pawar

Assistant Professor/ Assistant Director

School of Nanoscience and Biotechnology

Shivaji University,

Vidyanagari, Kolhapur, 416004

MS, India


Email: pawarkiran1912@gmail.com

Country of citizenship: India

Date of Birth: 1st June 1979.





Research Interests

  • Microbial Biotechnology:

  • Plant Biotechnology

  • Plant Molecular Biology.

  • Plant Tissue Culture

  • Phytochemistry

  • Nanobiotechnology



Academics
June 2014 to date: Working as Assistant Professor/ Assistant Director at School of Nanoscience and

Biotechnology, Shivaji University, Kolhapur.


Nov. 2012 – June 2014: Worked as Assistant Professor at Dept. of Biotechnology, Shivaji University, Kolhapur on “DBT-Shivaji University, Kolhapur- Interdisciplinary Programme on Life Science for Advanced Research and Education”.
June 2012-Nov. 2012: Worked as Dr. D. S. Kotahri Postdoc Fellow Div. of Biochemistry, Dept. of Chemistry, University of Pune,
June 2011-June 2012: Worked Assistant professor at Department of biotechnology, University of

Pune, India


2009-2011 Worked as DBT- Post Doctoral Fellow (Department of Biotechnology, Govt of India) at Molecular Biology Unit, Lab. # 3, National Centre for Cell Science, Pune, India, on topic “Molecular characterization of ESTs from Anopheles stephensi mosquitoes”.
2003-2009 Ph.D. in Biotechnology from National Chemical Laboratory, Pune, India registered under University of Pune, India. Title of my thesis was: “In vitro production of secondary metabolites from cultured cells/tissues and molecular characterization of Calophyllum inophyllum L”.
2000-2002 M. Sc. in Biotechnology from North Maharashtra University, Jalgaon, Maharashtra.

1997-2000 B. Sc. In botany from S. S. V. P. S. Science College, Dhule, Maharashtra affiliated to North Maharashtra University, Jalgaon, Maharashtra with Botany as principle subject.
Awards and Fellowship

  • Awarded Dr. D. S. Kothari Postdoctoral fellowship of UGC, India

  • Awarded Post Doctoral Fellowship of DBT (Department of Biotechnology, Govt. of India) for post doctoral studies in Molecular Biology.

  • Recipient of Senior Research Fellowship (SRF) of the Council of Scientific Industrial Research, India for continuing doctoral studies in 2005.

  • Qualified National Eligibility Test (NET, 2002) for Junior Research Fellowship/ Lectureship (JRF) of the Council of Scientific Industrial Research (CSIR-NET), India for pursuing doctoral studies in 2003.

  • Qualified SET (State Eligibility Test, Maharashtra for Lectureship) conducted by University of Pune, in 2003).


Laboratory Skills 

Molecular biology -PCR, RT-PCR, Gene cloning, Western Blotting, 51 and 31 RACE, Recombinant Protein expression and purification, Construction of genomic liabrary, EST liabrary, 16S rDNA liabrary, tRFLP, Sequencing. Establishment of molecular markers like RAPD, RFLP, SSR, ISSR for DNA fingerprinting.

Bioinformatics-Post sequencing analysis, sequence editing in ChromasPro, vector screen in Vec Screen (NCBI), BLAST search, orientation check in Mallard, sequence alignment in Clustal X/W and editing in DAMBE, statistical analysis in PHYLIP and DOTUR, and construction of phylogenetic tree in MEGA.

Microbiology Isolation and culturing of pure and recombinant microorganisms, Isolation of endophytic fungi, their identification, use of microorganisms as biotic elicitors.

Biochemistry and phytochemistry: Various phytochemical techniques like extraction, purification, column chromatography, TLC, Prep TLC, HPTLC, HPLC, Preparative HPLC, GC, and spectrophotometry.

Plant Tissue Culture: General plant tissue culture techniques, Callus Cultures, micropropagation, organogenesis, Cell suspension cultures, elicitation of cell suspension cultures. Hairy root cultures, transformation, secondary metabolite analysis. Histology techniques, Microscopy techniques.
Selected publications in last seven years in peer reviewed, international Journals.


  • Pawar K.D., Banskar, S., Rane S. D., Charan, S S., Sawant, S. S, Kulkarni G. J., Ghate H. V, Patole M. S., and Shouche Y.S. 2012 Bacterial diversity analysis in different regions of gastrointestinal tract of Giant African Snail, Achatina fulica. MicrobiologyOpen (In press).

  • Pawar, K. D. and S. R. Thengane. 2011. “Influence of abiotic elicitation on production of dipyranocoumarins in suspension cultures of Calophyllum inophyllum L.”. Acta Physiol. Planta. 33(6), 2146-2158.

  • Pawar, K. D. Yadav, A. V., Shouche, Y. S. and S. R. Thengane. 2011. “Influence of endophytic fungal elicitation on production of inophyllum in suspension cultures of Calophyllum inophyllum L”. Plant Cell Tiss. Organ Cult. 106 (2), 345-352

  • Pawar, K. D., Joshi, S. P., and Thengane, S. R. 2011. “Association between Chemical and Genetic variation in Calophyllum inophyllum, medicinally important tree of the Western Ghats of India.”. Plant Syst. Evol. 292, 257-265

  • Pawar, K. D. and Thengane, S. R. 2009. “Influence of hormones and medium components on expression of dipyranocoumarins in cell suspension cultures of Calophyllum inophyllum”. Process Biochem. 44, 916-922.

  • Pawar, K. D., Joshi, S. P. Bhide, S. R. and Thengane, S. R. 2007 “Pattern of anti- HIV dipyranocoumarins expression in callus cultures of Calophyllum inophyllum. J. Biotechnol. 130, 346-353.

  • Thengane, S. R., Bhosle, S. V., Deodhar., S. R., Pawar, K. D., Kulkarni, D. K. 2006 “Micropropagation of an Indian Laurel, Calophyllum inophyllum: source of anti HIV compounds”. Current Science. 90, 393-397.

  • Charan, S. S., Pawar, K. D, Severson, D. W., Patole, M. S., and Shouche, Y, S. “Comparative analysis of midgut bacterial communities of mosquitoes having differential abilities to transmit dengue viruses”. (accepted).


Papers under review/communication


  • Pawar, K. D., Gholave, A., Bapat, V. A., Yadav, S. R and Jadhav, J.P. 2014. Multiloccus DNA barcode for Phylogenetic analysis of Amorphophallus species of India. Journal of Integrative Plant Biology. (Under Review).

  • Dar, M. A., Pawar, K. D., Jadhav, J.P. and Pandit, R. S. 2014. Exploring and evaluation of GI tract of Achatina fulica as a source for isolation of cellulolytic bacteria.Research in Microbiology. (Under Review).

  • Pawar, K. D., Dar, M. A., Rajput, B.P. and Kulkarni, G.J. 2014. Enrichment and identification of cellulolytic bacteria from the gastrointestinal tract of Giant African Snail, Achatina fulica. Applied Biochemistry and Biotechnology. (Under Review).



Dr. Kiran D. Pawar


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